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T6666

Sigma

 

Trizma® hydrochloride

pH 3.5-5.0 (20 °C, 0.5 M in H2O), SigmaUltra, ≥99.0% (titration)

Synonym:TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride
CAS Number:1185-53-1
Linear Formula:NH2C(CH2OH)3 · HCl
Molecular Weight:157.60
Beilstein Registry Number:3675235
EC Number:214-684-5
MDL number:MFCD00012590
PubChem Substance ID:24900398

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Description

ApplicationThe pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other NotesEasily compare specifications for Trizma HCl products with the Trizma HCl specification table.
Legal InformationTrizma is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

gradeSigmaUltra
assay≥99.0% (titration)
total impurities Insoluble matter, passes filter test
ign. residue (900°C) ≤0.2% (as SO4)
loss≤0.2% loss on drying, 110°C
pH3.5-5.0 (20 °C, 0.5 M in H2O)
useful pH range7.0 - 9.0
pKa (25 °C)8.1
EQP levelElite
solubilityH2O: 0.5 M at 20 °C, clear, colorless
anion tracessulfate (SO42-): ≤0.005%
cation tracesAl: ≤0.0005%
 As: ≤0.0001%
 Ba: ≤0.0005%
 Bi: ≤0.0005%
 Ca: ≤0.001%
 Cd: ≤0.0005%
 Co: ≤0.0005%
 Cr: ≤0.0005%
 Cu: ≤0.0005%
 Fe: ≤0.0005%
 K: ≤0.005%
 Li: ≤0.0005%
 Mg: ≤0.0005%
 Mn: ≤0.0005%
 Mo: ≤0.0005%
 Na: ≤0.005%
 Ni: ≤0.0005%
 Pb: ≤0.0005%
 Sr: ≤0.0005%
 Zn: ≤0.0005%
abs.A0.5M/260, H2O ≤0.015
 A0.5M/280, H2O ≤0.010

Safety

Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Gloves
Hazard CodesXi
Risk Statements36/37/38
Safety Statements26-36
WGK Germany1

References

BeilsteinBeil. 4,H,304